This can be achieved by a combination of the feature to be quantified, the tissue preparation or the probe having isotropy. To achieve an unbiased result from stereologic analysis, the probe should interact with the feature to be quantified in an isotropic way. Q: Are you saying that, for any analysis to be possible, it needs to be isotropic? But keep in mind you will only be finding out about the biopsy, not about the whole region. But if you need to quantify it, you can use stereology. What are you trying to find out with the biopsy please? If it is something that you can find out about qualitatively, then there is no need for quantification. Q: I just wanted to add, about lung sterology, that we use small human lung biopsies about 2mm to be cut at 2 ยต m. Yes, it is relevant for any biological tissue you are viewing under the microscope. Q: Is the information presented in this webinar relevant for lung stereology?
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